Merck pSF-CMV-NEO-NH2-PPT-FLAG

pSF-CMV-NEO-NH2-PPT-FLAG

plasmid vector for molecular cloning

expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

재조합

expressed in human cells

태그

FLAG® tagged

form

buffered aqueous solution

분자량

size 5913 bp

박테리아 선정

ampicillin

복제개시점

pUC

펩타이드 절단

EKT

펩타이드 태그 위치

N-terminal

프로모터

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

리포터 유전자

none

배송 상태

ambient

저장 온도

−20°C

The pSF-CMV-NEO-NH2-PPT-FLAG expression vector is a 5.9 kb derivative of pSF-CMV-Amp for transient or stable expression of secreted N-terminal FLAG fusion proteins in mammalian cells. The FLAG epitope is a small- hydrophilic- 8 amino acid-tag (DYKDDDDK) that enables sensitive detection and high quality protein purification using anti-FLAG products such as the anti-FLAG M2 antibody (catalogue number F3165).The preprotrypsin (PPT) leader sequence precedes the FLAG sequence and directs secretion of the fusion protein into the culture medium. pSF-CMV-NEO-NH2-PPT-FLAG is a shuttle vector, containing both E. coli and SV40 origins of replication, for propagation in bacterial and mammalian cells. Efficiency of replication and genomic integration is optimal when using an SV40 T antigen expressing mammalian host. The promoter regulatory region of the human cytomegalovirus drives transcription of FLAG fusion constructs. The aminoglycoside phosphotransferase II gene (neo-R) confers resistance to aminoglycosides such as G418, allowing for selection of stable transfectants.