Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), eFluor 506, eBioscience
Human CD3 인식용 OKT3 클론 단일클론 항체로, eFluor 506 형광체가 결합된 Flow Cytometry 분석용 제품입니다. 고감도 신호 검출, 안정적 형광 특성, 면역학 연구 및 T 세포 활성화 분석에 적합합니다. 4°C 보관, 동결 금지.
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Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), eFluor 506, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 2 publications |
| Immunocytochemistry (ICC/IF) | - | View 2 publications |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 28 publications |
| Functional Assay | - | View 10 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), eFluor™ 506 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OKT3 |
| Conjugate | eFluor™ 506 |
| Excitation / Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2848507 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395, 563, 615, 661, 737, 805
- Brilliant Violet 421, 605
- eFluor 450, 660
- FITC
- Functional Grade
- NovaFluor Blue 510, 585
- NovaFluor Yellow 590, 660, 730
- PE, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation available
Product Specific Information
The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. It has potent immunosuppressive properties and is effective in treating renal, heart, and liver allograft rejection.
CD3 subunits (gamma, delta, epsilon) are essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Crosslinking of TCR initiates intracellular signaling leading to activation and proliferation.
Applications Reported: Flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometric analysis of normal human peripheral blood cells at 5 µL (0.25 µg)/test.
A test corresponds to staining a cell sample (10⁵–10⁸ cells) in 100 µL final volume.
Fluorophore Characteristics:
- Excitation: 405 nm
- Emission: 506 nm
- Recommended Filter: 510/20 band pass
- Laser: Violet Laser
Ensure instrument compatibility with eFluor 506 detection.
Target Information
CD3 complex is crucial for transducing antigen-recognition signals and regulating TCR surface expression.
It includes CD3 gamma, delta, epsilon, and zeta subunits, members of the immunoglobulin superfamily on chromosome 11.
CD3 is expressed on 68–82% of peripheral blood lymphocytes and thymocytes, absent on B or NK cells.
Defects in CD3 genes are linked to CD3 immunodeficiency.
May play roles in TCR-induced growth arrest, survival, and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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