Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), eFluor 506, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV / Violet / Blue / Yellow / Red series
• eFluor 450, 660
• FITC
• Functional Grade
• NovaFluor series (Blue, Red, Violet, Yellow, UV)
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation

Product Specific Information

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cell subpopulations, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays key roles in T cell development and activation. In T cells, CD4 associates with protein tyrosine kinase p56lck via its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported: Flow cytometric analysis.
Applications Tested: Validated in flow cytometry using mouse splenocytes at ≤0.125 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimal titration recommended.

Fluorochrome Information:
eFluor® 506 is excited by the 405 nm violet laser and emits at 506 nm. Recommended detection filter: 510/20 band pass or equivalent. Verify instrument compatibility.

Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-54)
  Clone-specific performance post-fixation should be empirically confirmed.

Excitation: 405 nm
Emission: 506 nm
Laser: Violet Laser

Target Information

CD4 antigen is a co-receptor for MHC class II molecules and HIV. It is mainly expressed on T helper cell subsets, as well as monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain brain and gut regions. CD4 aids early T-cell activation via association with TCR complex and Lck kinase, and may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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