Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), eFluor 506, eBioscience
마우스 CD4 분자를 인식하는 GK1.5 단클론 항체로, eFluor 506 형광체로 결합되어 있습니다. 유세포 분석(Flow Cytometry)에 적합하며, 405 nm 레이저로 여기되어 506 nm에서 방출됩니다. 4°C 암조건에서 보관하며, 동결 금지. 연구용으로만 사용 가능합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD4 Monoclonal Antibody (GK1.5), eFluor 506, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 6 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | 1 publication |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 0.125 µg/test | 90 publications |
Product Specifications
| Specification | Detail |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 506, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | GK1.5 |
| Conjugate | eFluor™ 506 |
| Excitation/Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2762799 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV / Violet / Blue / Yellow / Red series
- eFluor 450, 660
- FITC
- Functional Grade
- NovaFluor series (Blue, Red, Violet, Yellow, UV)
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436, 600, 645, 702, 780
- Request custom conjugation
Product Specific Information
The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by thymocytes, mature T cell subpopulations, and dendritic cells. CD4 binds to MHC class II molecules on antigen-presenting cells and plays key roles in T cell development and activation. In T cells, CD4 associates with protein tyrosine kinase p56lck via its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.
Applications Reported: Flow cytometric analysis.
Applications Tested: Validated in flow cytometry using mouse splenocytes at ≤0.125 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Optimal titration recommended.
Fluorochrome Information:
eFluor® 506 is excited by the 405 nm violet laser and emits at 506 nm. Recommended detection filter: 510/20 band pass or equivalent. Verify instrument compatibility.
Fixation:
Samples may be stored up to 3 days at 4°C in the dark using:
- IC Fixation Buffer (Product #00-8222-49)
- 1-step Fix/Lyse Solution (Product #00-5333-54)
Clone-specific performance post-fixation should be empirically confirmed.
Excitation: 405 nm
Emission: 506 nm
Laser: Violet Laser
Target Information
CD4 antigen is a co-receptor for MHC class II molecules and HIV. It is mainly expressed on T helper cell subsets, as well as monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and certain brain and gut regions. CD4 aids early T-cell activation via association with TCR complex and Lck kinase, and may also mediate neuronal damage in immune-related CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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