Thermo Fisher Scientific CD4 Monoclonal Antibody (RPA-T4), eFluor 506, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Unconjugated, Alexa Fluor 488, Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 496, Brilliant UV 615, Brilliant UV 661, Brilliant UV 737, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 480, Brilliant Violet 605, Brilliant Violet 650, Brilliant Violet 711, eFluor 450, FITC, Functional Grade, NovaFluor Blue 510, NovaFluor Blue 585, NovaFluor Blue 690, Pacific Orange, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, Super Bright 780.

Product Specific Information

The RPA-T4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface receptor expressed by most thymocytes, a subset of mature T cells (T-helper cells), and at low levels on monocytes. CD4 serves as a receptor for HIV. The RPA-T4 antibody blocks HIV binding and mixed lymphocyte reactions. It recognizes a different epitope than the OKT4 monoclonal antibody, and the two do not cross-block each other.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and validated for flow cytometry of normal human peripheral blood cells. Recommended usage: 5 µL (0.25 µg) per 100 µL test volume, with 10⁵–10⁸ cells/test.
• Fluorochrome Information: eFluor® 506 is excited by the 405 nm violet laser and emits at 506 nm. Recommended detection filter: 510/20 band pass or equivalent.
• Filtration: 0.2 µm post-manufacturing filtered.

Excitation: 405 nm
Emission: 506 nm
Laser: Violet

Target Information

CD4 is involved in the recognition of MHC class II molecules and acts as a co-receptor for HIV. It is primarily expressed on T-helper cells, and also found on monocytes, macrophages, and dendritic cells. Tissue expression includes thymus, lymph nodes, tonsils, spleen, and specific regions of brain and gut. CD4 mediates early T-cell activation via association with the T-cell receptor complex and protein tyrosine kinase Lck. It may also contribute to neuronal damage in infectious and immune-mediated CNS diseases. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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