Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), eFluor 506, eBioscience
인간 CD3e를 인식하는 UCHT1 클론의 단클론 항체로, eFluor 506 형광체가 결합되어 있습니다. 유세포분석(Flow Cytometry)에 최적화되어 있으며, T세포 표면 CD3 복합체 분석에 사용됩니다. 405 nm 레이저로 여기되며 506 nm에서 방출됩니다.
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- 69-0038-4x (2개 옵션)
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Thermo Fisher Scientific CD3 Monoclonal Antibody (UCHT1), eFluor 506, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 3 publications |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.5 µg)/test | View 25 publications |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 506, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | UCHT1 |
| Conjugate | eFluor™ 506 |
| Excitation / Emission Max | 419 / 508 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2637488 |
Available Formats
- Unconjugated
- Alexa Fluor 532, 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV, Violet, Yellow series
- FITC, PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- Super Bright 436, 600, 645, 702, 780
- NovaFluor Blue, Red, Violet, Yellow series
- Functional Grade
- Request custom conjugation
Product Specific Information
The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain, along with gamma and delta subunits, is essential for proper assembly and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells.
Crosslinking of TCR via immobilized UCHT1 initiates intracellular signaling leading to activation and proliferation.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated and validated for flow cytometry using normal human peripheral blood cells
- Recommended Use: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)
- Fluorochrome: eFluor® 506 (excitation 405 nm, emission 506 nm)
- Recommended Filter: 510/20 band pass or equivalent
- Filtration: 0.2 µm post-manufacturing filtered
Target Information
The CD3 complex is crucial for transmitting antigen-recognition signals in T cells and regulating TCR surface expression. It consists of subunits CD3γ, CD3δ, CD3ε, and CD3ζ, encoded on chromosome 11. These subunits have cytoplasmic tails associated with signaling molecules through tyrosine-based motifs.
CD3 is expressed on:
- 68–82% of normal peripheral blood lymphocytes
- 65–85% of thymocytes
- Purkinje cells in the cerebellum
Not expressed on B or NK cells. CD3 defects are linked to immunodeficiency and altered T cell percentages in autoimmune diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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