Thermo Fisher Scientific GL7 Monoclonal Antibody (GL-7 (GL7)), PE, eBioscience

Applications

Flow Cytometry (Flow)

• Tested Dilution: 0.25 µg/test
• View 17 publications

Functional Assay (Functional)

• Tested Dilution: –
• View 1 publication

Product Specifications

Additional Formats

• Unconjugated
• Alexa Fluor 488
• Biotin
• eFluor 450
• eFluor 660
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

This GL7 monoclonal antibody reacts with a cell-surface protein found on T and B lymphocytes activated in vitro, on bone marrow pre-B-II cells, germinal center B cells, and also human B cell lines Ramos and Daudi. There is strain variability with respect to antigen distribution on thymocytes and Con A-activated spleen cells, with expression in BALB/c greater than that in C57BL/6. GL7 is commonly used as a marker for mouse germinal center B cells. The epitope of GL7 has been identified as a sialic acid glycan moiety called Neu5Ac, which is recognized by CD22.

Applications Reported:

• Flow cytometric analysis.

Applications Tested:

• Tested by flow cytometric analysis of stimulated mouse splenocytes.
• Recommended ≤0.25 µg/test (in 100 µL final volume).
• Cell number: 10⁵–10⁸ cells/test.
• Optimize by titration for best performance.

Excitation / Emission / Laser:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green

Filtration:

• 0.2 µm post-manufacturing filtered.

Target Information

GL7 is a cell-surface protein found on activated T and B lymphocytes, bone marrow pre-B-II cells, germinal center B cells, and human B cell lines (Ramos, Daudi). Expression varies by strain, higher in BALB/c than C57BL/6. GL7 is a marker for mouse germinal center B cells. The epitope is a sialic acid glycan moiety (Neu5Ac) recognized by CD22.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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