Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (MFL3), PE, eBioscience
마우스 Fas Ligand(CD178)을 인식하는 단클론 항체로, PE 형광 표지되어 Flow Cytometry에 적합합니다. 활성화된 T 세포 분석에 사용되며, 488–561 nm 레이저에서 최적의 형광 특성을 가집니다. 연구용으로만 사용 가능합니다.
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- 12-5911-8x (2개 옵션)
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- pk
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD178 (Fas Ligand) Monoclonal Antibody (MFL3), PE, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 17 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse, Rat |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MFL3 |
| Conjugate | PE |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_466038 |
Available Formats
- APC
- APC-eFluor 780
- Biotin
- FITC
- Functional Grade
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 600
- Request custom conjugation
Product Specific Information
The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family expressed by activated T cells. Interaction of FasL with receptor CD95 induces Fas-mediated apoptosis. Human FasL can be cleaved by MMPs to produce a 26 kDa soluble form, though mouse sensitivity to MMPs is not reported.
Applications Reported: Flow cytometric analysis.
Applications Tested: Tested on mouse FasL transfected and activated T cells. Recommended ≤1 µg/test (in 100 µL volume, 10⁵–10⁸ cells/test). Optimize concentration for best performance.
Fluorescence Characteristics:
Excitation: 488–561 nm
Emission: 578 nm
Laser Compatibility: Blue, Green, Yellow-Green Lasers
Filtration: 0.2 µm post-manufacturing filtered
Target Information
CD178 (Fas ligand, FasL) is a type-II membrane protein with its N-terminus in the cytoplasm and C-terminus extracellular. Its receptor, FasR, belongs to the TNF/NGF receptor family. CD178 induces apoptosis upon binding to FasR and exists as membrane-bound (45 kDa) or soluble (26 kDa) forms, the latter released by metalloproteinase cleavage. CD178 is predominantly expressed in activated T cells and NK cells, and found in immune-privileged tissues such as testis and eye. It also appears in thymus, liver, ovary, lung, heart, and kidney. The Fas/CD178 system plays a role in immune regulation, cytotoxicity, and diseases including AIDS, hepatitis, cancer, and inflammatory disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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