Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PE, eBioscience

Thermo Fisher Scientific FceR1 alpha Monoclonal Antibody (AER-37 (CRA1)), PE, eBioscience

Applications

• Flow Cytometry (Flow)
  Tested dilution: 5 µL (0.25 µg)/test
  View 12 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant Violet 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Yellow 570
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 780
• Request custom conjugation

Product Specific Information

The AER-37 monoclonal antibody reacts with the Fc epsilon RI alpha subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRI alpha is expressed on mast and basophil cells and is upregulated by the presence of IgE. It forms a tetrameric complex with one beta and two gamma subunits, which possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex is critical in triggering IgE-mediated allergic responses.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated and tested by flow cytometry using peripheral blood cells
  • Use 5 µL (0.25 µg) per test (100 µL final volume)
  • Cell number: 10⁵–10⁸ cells/test (empirically determined)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser Compatibility: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

FceR1 (Fc epsilonR1 alpha) is a subunit of the high-affinity receptor for IgE. It forms a tetrameric complex (αβγ₂) where the β and γ subunits mediate signal transduction. This receptor plays a key role in allergic responses by coupling allergen-bound IgE to mast cells, leading to histamine and protease release, and synthesis of prostaglandins and leukotrienes—potent mediators of hypersensitivity reactions such as hay fever and asthma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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