Thermo Fisher Scientific CD137 Ligand (4-1BB Ligand) Monoclonal Antibody (TKS-1), PE, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1 µg/test
• Publications: View 4 publications

Product Specifications

Available Formats

• Biotin
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor (TNF) superfamily. 4-1BBL is expressed by activated antigen-presenting cells, including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) delivers a co-stimulatory signal important in T cell–APC interaction.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on resting and 2–3 day (IgM+CD40)-activated mouse splenocytes. Recommended ≤1 µg per test (100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Antibody should be titrated for optimal performance.

Fluorescence Characteristics:

• Excitation: 488–561 nm
• Emission: 578 nm
• Compatible Lasers: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

CD137 ligand (CD137L) is a type II membrane protein and part of the TNF superfamily. It is a co-stimulatory molecule expressed on antigen-presenting cells (DCs, monocytes/macrophages, B cells) and upregulated upon activation. Its receptor, CD137 (4-1BB), is found on various cells including inflamed endothelial cells and activated T cells. Engagement of CD137 with CD137L enhances T cell proliferation, IL-2 secretion, survival, and cytotoxic activity.

CD137L is expressed on most B-cell lymphomas (mantle cell, follicular, diffuse large B-cell) but absent in Hodgkin and T-cell lymphomas. It is also found in carcinoma cell lines, suggesting a role in T cell–tumor cell interactions.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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