Thermo Fisher Scientific Phospho-ROCK1 (Tyr913) Polyclonal Antibody
ROCK1 단백질의 Tyr913 인산화 형태를 특이적으로 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 적합. Human, Mouse, Rat 반응성. 고순도 액상 형태로 연구용으로 제공.
- 카탈로그번호
- PA5105054
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- pk
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human ROCK1 (Accession Q13464), corresponding to amino acid residues around phosphorylated Tyr913 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816527 |
Product Specific Information
This antibody detects endogenous levels of ROCK1 only when phosphorylated at Tyr913.
Target Information
ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been identified as a Caspase-3 substrate. It contributes to myosin light chain (MLC) activation through direct phosphorylation and inhibition of MLC phosphatase, leading to increased cellular contractility required for apoptotic membrane blebbing.
ROCK1 consists of an amino-terminal kinase domain and a cysteine/histidine-rich C-terminal domain within a pleckstrin-homology region, connected by a variable region containing the Rho-binding domain. During apoptosis, ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence, removing its inhibitory domain and resulting in constitutive kinase activity. This cleavage induces MLC phosphorylation and membrane blebbing, key events in apoptosis execution.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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