Thermo Fisher Scientific Phospho-ROCK1 (Tyr913) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

This antibody detects endogenous levels of ROCK1 only when phosphorylated at Tyr913.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been identified as a Caspase-3 substrate. It contributes to myosin light chain (MLC) activation through direct phosphorylation and inhibition of MLC phosphatase, leading to increased cellular contractility required for apoptotic membrane blebbing.

ROCK1 consists of an amino-terminal kinase domain and a cysteine/histidine-rich C-terminal domain within a pleckstrin-homology region, connected by a variable region containing the Rho-binding domain. During apoptosis, ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence, removing its inhibitory domain and resulting in constitutive kinase activity. This cleavage induces MLC phosphorylation and membrane blebbing, key events in apoptosis execution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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