Thermo Fisher Scientific Phospho-ROCK1 (Ser282) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of ROCK1 only when phosphorylated at Ser282.

Target Information

ROCK1 is a serine/threonine kinase that regulates cell differentiation and migration. The Rho/ROCK pathway is involved in the progression of various human cancers. ROCK1 has been found to be a new Caspase-3 substrate. ROCK, one of the effectors of the small GTPase Rho, contributes to myosin light chain (MLC) activation through direct phosphorylation of MLC and phosphorylation of MLC phosphatase, leading to its inhibition. The increase in cellular contractility necessary for apoptotic membrane blebbing implies sustained augmentation of MLC phosphorylation. ROCK1 consists of an amino-terminal kinase domain and an inhibitory cysteine/histidine-rich C-terminal domain within a pleckstrin-homology region, joined by a variable region containing the Rho-binding domain. During apoptosis, ROCK1 is cleaved by caspase-3 at a conserved DETD1113/G sequence, removing its inhibitory domain and resulting in constitutive kinase activity. This cleavage and activation induce phosphorylation of MLC and membrane blebbing, an early event in apoptosis execution.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.