Thermo Fisher Scientific C19orf10 Polyclonal Antibody, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine SF-20. Anti-Murine SF-20-specific antibody was purified by affinity chromatography employing an immobilized Murine SF-20 matrix.

Sandwich ELISA:
To detect Murine SF-20 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. In conjunction with PeproTech Biotinylated Anti-Murine SF-20 (500-P259Bt) as a detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Murine SF-20.

Western Blot:
To detect Murine SF-20 by Western Blot analysis, use at a concentration of 0.1–0.2 µg/mL. When used with compatible secondary reagents, the detection limit for Recombinant Murine SF-20 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Target Information

The interleukins are a broad family of well-characterized cytokines, primarily of hematopoietic cell origin. Cytokines are small, soluble proteins with pleiotropic effects on various cell types and play regulatory roles in the immune system and inflammatory responses.
IL-25 (also known as SF20/IL-25) is a secreted bone marrow stroma-derived growth factor cytokine from Th2 T-cells. It belongs to the IL-17 cytokine family and amplifies allergic-type inflammatory responses. IL-25 binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation.
Infusion of mice with IL-25 induces IL-4, IL-5, and IL-13 gene expression, leading to epithelial cell hyperplasia, increased mucus secretion, and airway hyperreactivity—suggesting IL-25’s role as a key mediator of allergic diseases.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.