Thermo Fisher Scientific TSLP Polyclonal Antibody, PeproTech
Human TSLP를 인식하는 Rabbit Polyclonal Antibody로, Western Blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 동결건조 형태로 제공됩니다. 인체 면역 및 염증 연구용으로 활용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA | 0.5–2.0 µg/mL | – |
| In vitro Assay (IV) | – | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human TSLP |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930110 |
Product Specific Information
AA Sequence of recombinant protein:
MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TSLP. Anti-Human TSLP-specific antibody was purified by affinity chromatography employing an immobilized Human TSLP matrix.
Sandwich ELISA:
To detect Human TSLP by sandwich ELISA (100 µL/well antibody solution), use 0.5–2.0 µg/mL of this antibody. When paired with PeproTech Biotinylated Anti-Human TSLP (500-P258Bt) as detection antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human TSLP.
Western Blot:
For detection of Human TSLP by Western Blot, use 0.1–0.2 µg/mL of this antibody. With compatible secondary reagents, detection limit for Recombinant Human TSLP is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
TSLP is a 159 amino acid four-helix bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells, induces T cell-attracting chemokine release, enhances dendritic cell maturation, and influences T cell homeostasis. TSLP may contribute to allergic inflammation and inflammatory arthritis and is expressed in heart, liver, and prostate.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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