Thermo Fisher Scientific TSLP Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human TSLP. Anti-Human TSLP-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hTSLP by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human TSLP (500-P258) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hTSLP.

Western Blot:
To detect hTSLP by Western blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hTSLP is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P258BT-1MG will be provided as 2 × 500 µg.

Target Information

TSLP is a 159 amino acid four-helix bundle hemopoietic cytokine that signals through a heterodimeric receptor complex consisting of the human TSLP receptor and the IL-7R alpha-chain. It impacts myeloid cells and induces the release of T cell-attracting chemokines from monocytes, enhances the maturation of CD11c(+) dendritic cells, and drives naive CD4(+) and CD8(+) T cells into proallergic effectors. TSLP primes dendritic cells to produce large amounts of IL-12 after CD40 ligand stimulation, regulates the positive selection of regulatory T cells, and maintains peripheral CD4(+) T cell homeostasis. It induces allergic inflammation by directly activating mast cells and may be involved in inflammatory arthritis. Expression is observed in heart, liver, and prostate.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.