Thermo Fisher Scientific C19orf10 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine SF-20. Anti-Murine SF-20-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mSF-20 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SF-20 (500-P259) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mSF-20.

Western Blot:
To detect mSF-20 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant mSF-20 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package:
500-P259BT-1MG is provided as 2 × 500 µg.

Target Information

Interleukins are a broad family of cytokines, primarily of hematopoietic cell origin.
Cytokines are small, soluble proteins with pleiotropic effects on various cell types and play regulatory roles in immune system modulation and inflammatory responses.

IL-25 (also called SF20/IL-25):

• Secreted bone marrow stroma-derived growth factor cytokine derived from Th2 T-cells
• Member of the IL-17 cytokine family
• Amplifies allergic-type inflammatory responses
• Binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation
• Induces IL-4, IL-5, and IL-13 gene expression in mice
• Causes epithelial hyperplasia, increased mucus secretion, and airway hyperreactivity

These findings suggest that IL-25 may be an important mediator of allergic disease via production of IL-4, IL-5, IL-13, and eotaxin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.