Thermo Fisher Scientific C19orf10 Polyclonal Antibody, Biotin, PeproTech
Biotin으로 표지된 C19orf10(IL-25/SF-20) 폴리클로날 항체로, 마우스 시료에서 Western blot 및 ELISA에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, 높은 특이성과 민감도를 제공합니다. -20°C에서 보관하며 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P259BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific C19orf10 Polyclonal Antibody, Biotin, PeproTech
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL |
| ELISA | 0.25–1.0 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived, 15.8 kDa Recombinant Murine SF-20 |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930120 |
Product Specific Information
Amino Acid Sequence of Recombinant Protein:
MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Murine SF-20. Anti-Murine SF-20-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect mSF-20 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine SF-20 (500-P259) as a capture antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant mSF-20.
Western Blot:
To detect mSF-20 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant mSF-20 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.
Package:
500-P259BT-1MG is provided as 2 × 500 µg.
Target Information
Interleukins are a broad family of cytokines, primarily of hematopoietic cell origin.
Cytokines are small, soluble proteins with pleiotropic effects on various cell types and play regulatory roles in immune system modulation and inflammatory responses.
IL-25 (also called SF20/IL-25):
- Secreted bone marrow stroma-derived growth factor cytokine derived from Th2 T-cells
- Member of the IL-17 cytokine family
- Amplifies allergic-type inflammatory responses
- Binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation
- Induces IL-4, IL-5, and IL-13 gene expression in mice
- Causes epithelial hyperplasia, increased mucus secretion, and airway hyperreactivity
These findings suggest that IL-25 may be an important mediator of allergic disease via production of IL-4, IL-5, IL-13, and eotaxin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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