Thermo Fisher Scientific C/EBP beta Monoclonal Antibody (1H7)
C/EBP beta 단백질의 LAP*, LAP, LIP isoform을 인식하는 mouse monoclonal antibody. Western blot, ICC/IF, IP, ChIP 등 다양한 응용에 적합. Recombinant mouse LIP 면역원으로 제작되었으며, Protein G로 정제된 액상 형태로 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1 µg/mL | View 4 publications |
| Immunocytochemistry (ICC/IF) | 4 µg/mL | - |
| Immunoprecipitation (IP) | Assay-dependent | View 2 publications |
| ChIP assay (ChIP) | - | View 1 publication |
| Gel Shift (GS) | - | View 1 publication |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Mouse, Rat |
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H7 |
| Immunogen | Recombinant mouse LIP |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2078054 |
Product Specific Information
MA1-827 detects C/EBP beta protein in mouse and rat samples. It recognizes the LAP*, LAP, and LIP isoforms of C/EBP beta.
This antibody has been validated for use in Western blot, immunofluorescence, and immunoprecipitation assays.
In Western blot analysis, MA1-827 detects 38, 35, and 20 kDa proteins corresponding to C/EBP beta LAP*, LAP, and LIP isoforms in 3T3-L1 cells. The LIP isoform represents the C-terminal fragment.
Immunofluorescent staining of C/EBP beta in P388D1 cells can be performed using MA1-827.
The immunogen used is recombinant mouse LIP.
Target Information
This gene belongs to the forkhead family of transcription factors, characterized by a distinct DNA-binding forkhead domain. Its specific biological function remains unclear, but it may play a role in mesenchymal tissue development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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