Thermo Fisher Scientific CD8 Monoclonal Antibody (12.C7)

Applications

Immunohistochemistry (IHC)

• Tested dilution: Not specified
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Flow Cytometry (Flow)

• Tested dilution: 1:100–1:200
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Product Specifications

Additional Formats

• FITC-conjugated format (MA1-83219)
• Request custom conjugation

Product Specific Information

For FACS analysis, use 10 µL of the suggested working dilution to label 1×10⁶ cells in 100 µL.

Mouse anti-Rabbit CD8 antibody, clone 12.C7 recognizes the rabbit CD8 cell surface antigen, expressed by a subset of T lymphocytes with cytotoxic/suppressor activity.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein of the immunoglobulin receptor family, playing an integral role in signal transduction and T cell differentiation and activation.
CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8α and CD8β, functioning as a co-receptor with the T cell receptor (TCR) for MHC class I molecules.
It is also expressed on subsets of T cells, NK cells, monocytes, and dendritic cells as CD8α homodimers.
Engagement of MHC-I/peptide complexes triggers recruitment of Lck kinase, leading to lymphokine production and cytotoxic T lymphocyte (CTL) activation.
Activated CTLs play a crucial role in pathogen and tumor cell clearance. Differentiation of naïve CD8⁺ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.