Thermo Fisher Scientific CD68 Monoclonal Antibody (Ki-M7), FITC

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100

-

Flow Cytometry (Flow)

Assay-dependent

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Ki-M7

Immunogen

Cell suspensions of human lymph node. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD68,uniProtId:P34810-1,ncbiNodeId:9606,antigenRange:1-354,antigenLength:354,antigenImageFileName:MA1-82715_CD68_P34810-1_House_mouse.svg,antigenImageFileNamePDP:MA1-82715_CD68_P34810-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein A

Storage buffer

PBS with 1% BSA

Contains

0.05% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_929275

Product Specific Information

Membrane permeabilization is required for flow cytometry applications. For FACS analysis, use 20 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD68 antibody, clone Ki-M7 recognizes the 110KD integral membrane glycoprotein CD68 (Macrosialin) predominantly expressed on the intracellular lysosomes of macrophages/monocytes, including Kupffer cells, microglia, histiocytes and osteoclasts, and is expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.

Target Information

CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Also, CD68 could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. CD68 is expressed by interdigitating reticulum cells in tonsil and some histiocytic lymphoma or histiocytosis, acute myeloid leukemia (AML), and granulocytic sarcoma. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several Acute Myeloid Leukemia studies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.