Thermo Fisher Scientific CD68 Monoclonal Antibody (Ki-M7), FITC
CD68 단백질을 인식하는 Ki-M7 클론의 Mouse Monoclonal Antibody로 FITC 형광 표지. 인간 세포에 반응하며 IHC, ICC/IF, Flow Cytometry에 사용 가능. 단백질 A 정제, PBS + 1% BSA buffer, 4°C 암소 보관.
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Thermo Fisher Scientific CD68 Monoclonal Antibody (Ki-M7), FITC
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| Flow Cytometry (Flow) | Assay-dependent | View 3 publications |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | Ki-M7 |
| Immunogen | Cell suspensions of human lymph node |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | Not Determined |
| Purification | Protein A |
| Storage Buffer | PBS with 1% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_929275 |
Product Specific Information
- Membrane permeabilization is required for flow cytometry applications.
- For FACS analysis, use 20 µL of the suggested working dilution to label 1×10^6 cells in 100 µL.
- Mouse anti-Human CD68 antibody, clone Ki-M7 recognizes the 110 kDa integral membrane glycoprotein CD68 (Macrosialin), predominantly expressed on intracellular lysosomes of macrophages/monocytes (Kupffer cells, microglia, histiocytes, osteoclasts).
- Also expressed to a lesser extent by dendritic cells and peripheral blood granulocytes.
Target Information
CD68 (Macrosialin) is a 110 kDa integral membrane glycoprotein predominantly expressed on intracellular lysosomes of monocytes and macrophages and to a lesser extent by dendritic cells and granulocytes.
It plays a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular interactions.
CD68 is expressed by interdigitating reticulum cells in tonsil and in certain histiocytic lymphoma, histiocytosis, AML, and granulocytic sarcoma.
Elevated CD68 expression has been observed in CD34+ cells in various human malignancies, including Acute Myeloid Leukemia studies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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