Thermo Fisher Scientific IL-10 Monoclonal Antibody (JES3-12G8)

Applications

Tested Dilution

Publications

Western Blot (WB)

1/50-1/250

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1/50 - 1/100

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Bacteria, Human

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

JES3-12G8

Immunogen

Human IL-10 expressed by COS cells.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_934131

Product Specific Information

Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for this product is human tonsil.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti Human Interleukin-10 antibody, clone JES3-12G8 recognizes human Interleukin-10 (IL-10).

Target Information

IL-10 encodes a protein that acts as a cytokine and is primarily produced by monocytes, with some production by lymphocytes. This cytokine has various effects on immunoregulation and inflammation, including down-regulating the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. Additionally, IL-10 can block NF-kappa B activity and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice have suggested that this cytokine is an essential immunoregulator in the intestinal tract. Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. Diseases associated with IL10 include Graft-Versus-Host Disease and Human Immunodeficiency Virus Type 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.