Thermo Fisher Scientific RIP2 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

• Recommended positive controls: HeLa, 293T, A431, H1299, HeLaS3, HepG2, Raji
• Predicted reactivity: Bovine (80%)
• Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

RIPK2 is a serine/threonine kinase involved in cell activation, proliferation, differentiation, and apoptosis. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through CARD-CARD domains. RIPK2 contributes to tyrosine phosphorylation of ARHGEF2 via Src kinase, leading to NF-κB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes Lys-63-linked polyubiquitination by E3 ligases XIAP, BIRC2, and BIRC3, mediating recruitment of MAP3K7/TAK1 to IKBKG/NEMO and subsequent NF-κB activation. This pathway regulates immune response, growth control, and apoptosis protection. RIPK2 also promotes BCL10 phosphorylation during T-cell receptor engagement, enhancing NF-κB activation.

Safety Information

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.