Thermo Fisher Scientific CXCL13 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BCA-1 (CXCL13). Anti-Human BCA-1 (CXCL13)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human BCA-1 (CXCL13) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BCA-1 (CXCL13) (500-P141) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BCA-1 (CXCL13).

Western Blot:
To detect hBCA-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBCA-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P141BT-1MG will be provided as 2 × 500 µg.

Target Information

CXCL13 (also known as BCA-1 and Angie) is a small chemokine strongly expressed in secondary lymphoid organs. It promotes migration of B lymphocytes to follicles of the spleen, lymph nodes, and Peyer’s patches. Produced by macrophages, high expression is found in large peritoneal macrophages, Kupffer cells, and lung macrophages.
The cellular ligand for CXCL13 is CXCR5 (BLR-1), expressed on B cells, Tfh cells, and some memory T cells. CXCL13 plays a role in chronic lymphocytic leukemia (CCL) development and serves as a plasma biomarker for germinal center activity and broadly neutralizing antibody generation against HIV.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.