Thermo Fisher Scientific CXCL13 Polyclonal Antibody, Biotin, PeproTech
CXCL13(BCA-1) 단백질을 인식하는 Biotin 결합 폴리클로날 항체로, Human 시료에 반응합니다. ELISA 및 Western Blot에 최적화되어 있으며, 항원 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용 가능합니다.
- 카탈로그번호
- 500-P141BT-xxxx (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CXCL13 Polyclonal Antibody, Biotin, PeproTech
Applications
Western Blot (WB)
- Tested Dilution: 0.1–0.2 µg/mL
ELISA
- Tested Dilution: 0.25–1.0 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Human BCA-1 (CXCL13) |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS |
| Contains | No preservative |
| Storage conditions | -20°C |
| Shipping conditions | Ambient |
| RRID | AB_2929457 |
Product Specific Information
AA Sequence of recombinant protein:
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BCA-1 (CXCL13). Anti-Human BCA-1 (CXCL13)-specific antibody was purified by affinity chromatography and then biotinylated.
Sandwich ELISA:
To detect Human BCA-1 (CXCL13) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BCA-1 (CXCL13) (500-P141) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BCA-1 (CXCL13).
Western Blot:
To detect hBCA-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBCA-1 is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.
Packaging:
500-P141BT-1MG will be provided as 2 × 500 µg.
Target Information
CXCL13 (also known as BCA-1 and Angie) is a small chemokine strongly expressed in secondary lymphoid organs. It promotes migration of B lymphocytes to follicles of the spleen, lymph nodes, and Peyer’s patches. Produced by macrophages, high expression is found in large peritoneal macrophages, Kupffer cells, and lung macrophages.
The cellular ligand for CXCL13 is CXCR5 (BLR-1), expressed on B cells, Tfh cells, and some memory T cells. CXCL13 plays a role in chronic lymphocytic leukemia (CCL) development and serves as a plasma biomarker for germinal center activity and broadly neutralizing antibody generation against HIV.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.