Thermo Fisher Scientific CXCL13 Polyclonal Antibody, Biotin, PeproTech

Thermo Fisher Scientific CXCL13 Polyclonal Antibody, Biotin (PeproTech)

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of Recombinant Protein:
VLEVYYTSLR CRCVQESSVF IPRRFIDRIQ ILPRGNGCPR KEIIVWKKNK SIVCVDPQAE WIQRMMEVLR KRSSSTLPVP VFKRKIP

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human BCA-1 (CXCL13). Anti-Human BCA-1 (CXCL13)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human BCA-1 (CXCL13) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human BCA-1 (CXCL13) (500-P141) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human BCA-1 (CXCL13).

Western Blot:
To detect hBCA-1 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hBCA-1 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Package Information:
500-P141BT-1MG will be provided as 2 × 500 µg.

Target Information

CXCL13, also known as BCA-1 and Angie, is a small chemokine strongly expressed in secondary lymphoid organs. It promotes the migration of B lymphocytes (B-1 and B-2) to follicles of the spleen, lymph nodes, and Peyer’s patches. It is produced by macrophages, including large peritoneal macrophages, Kuppfer cells, and lung macrophages (CD11c+ and CD11c−).
The cellular ligand for CXCL13 is CXCR5 (BLR-1), expressed on B cells, Tfh cells, and some memory T cells. CXCL13 plays an important role in the development of chronic lymphocytic leukemia (CCL) and serves as a plasma biomarker for germinal center activity and broadly neutralizing antibody generation against HIV.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.