Thermo Fisher Scientific TNFR1 (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MDSVCPQGKY IHPQNNSICC TKCHKGTYLY NDCPGPGQDT DCRECESGSF TASENHLRHC LSCSKCRKEM GQVEISSCTV DRDTVCGCRK NQYRHYWSEN LFQCFNCSLC LNGTVHLSCQ EKQNTVCTCH AGFFLRENEC VSCSNCKKSL ECTKLCLPQI EN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human sTNF Receptor Type I. Anti-Human sTNF Receptor Type I-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human sTNF Receptor Type I by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human sTNF Receptor Type I (500-P143) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human sTNF Receptor Type I.

Western Blot:
To detect hsTNF-Receptor I by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used with compatible secondary reagents, the detection limit for Recombinant hsTNF-Receptor I is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P143BT-1MG will be provided as 2 × 500 µg.

Target Information

TNFR1 (Tumor necrosis factor receptor 1) belongs to the tumor necrosis factor superfamily and is one of the major TNF-alpha receptors. TNFR1 plays an important role in cytokine-mediated signaling, positive regulation of the NF-κB pathway, angiogenesis, and negative regulation of gene expression.
The extracellular domain of TNFR1 is released into the circulatory system as soluble TNFR1 (sTNFR1). In humans, the TNFR1 gene is located on chromosome 12.
Proteins such as BAG4/SODD, TRADD, and TRAF2 interact with TNFR1 and regulate its signal transduction. Germline mutations in the extracellular domains of TNFR1 are associated with autosomal dominant periodic fever syndrome, likely due to impaired receptor clearance.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.