Thermo Fisher Scientific CD154 (CD40 Ligand) (soluble) Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

Amino Acid Sequence of Recombinant Protein:
MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Human sCD40 Ligand. Anti-Human sCD40 Ligand-specific antibody was purified by affinity chromatography and subsequently biotinylated.

Sandwich ELISA:
For detection of Human sCD40 Ligand by sandwich ELISA (100 µL/well antibody solution), use 0.25–1.0 µg/mL of this antibody.
When used with PeproTech Polyclonal Anti-Human sCD40 Ligand (500-P142G) as a capture antibody, it allows detection of at least 0.2–0.4 ng/well of Recombinant Human sCD40 Ligand.

Western Blot:
For detection of hsCD40L by Western Blot, use at 0.1–0.2 µg/mL.
When used with compatible secondary reagents, the detection limit for Recombinant hsCD40L is 1.5–3.0 ng/lane under reducing or non-reducing conditions.

Packaging:
500-P142GBT-1MG is supplied as 2 × 500 µg.

Target Information

CD40 Ligand (CD40-L, CD154) is a membrane glycoprotein and differentiation antigen expressed on T cells. It stimulates B cell proliferation and immunoglobulin secretion in the presence of cytokines. CD40 Ligand also induces cytokine production and tumoricidal activity in monocytes and co-stimulates proliferation of activated T cells, accompanied by production of IFN-γ, TNF-α, and IL-2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.