Thermo Fisher Scientific Bcl-2 Recombinant Rabbit Monoclonal Antibody (BCL2, 6426R)
Bcl-2 단백질을 표적하는 Thermo Fisher Scientific의 재조합 토끼 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 고순도 Protein A/G 정제, HEK293 발현, PBS 기반 저장용액 포함.
- 카탈로그번호
- 596-RBM15-P1
- 판매단위
- pk
카탈로그
1개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Bcl-2 Recombinant Rabbit Monoclonal Antibody (BCL2, 6426R)
Thermo Fisher Scientific Bcl-2 Recombinant Rabbit Monoclonal Antibody (BCL2, 6426R)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BCL2, 6426R |
| Immunogen | Synthetic peptide, aa41–54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
BCL-2 is a key regulator of apoptosis that can either inhibit or promote cell death. Members of the BCL-2 family dimerize to modulate apoptosis. Bag1 forms heterodimers with BCL-2 to enhance anti-apoptotic effects, while Bax and Bak promote cytochrome c release from mitochondria, initiating apoptosis. Constitutive expression of BCL-2, such as through translocation to the Ig heavy chain locus, is associated with follicular lymphoma. Neoplastic germinal centers in follicular lymphomas express high levels of BCL-2 alpha protein, whereas normal germinal centers do not. Overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer. Two transcript variants of BCL-2 differ at their C-terminal ends due to alternative splicing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(이미지 없음)
Thermo Fisher Scientific 상품 둘러보기
전체보기
문의
0개 · 배송·재고 문의는 실시간 상담이 빠릅니다아직 등록된 문의가 없어요.