Thermo Fisher Scientific Bcl-2 Recombinant Rabbit Monoclonal Antibody (BCL2, 2210R)
Bcl-2 단백질을 표적하는 Thermo Fisher의 재조합 토끼 단클론 항체로, Western blot 및 IHC(P) 등 다양한 응용에 사용 가능. 인간 시료에 반응하며, HEK293 발현 시스템에서 생산된 IgG 형식의 항체. 단백질 A/G로 정제된 액상 형태로 제공.
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- 596-RBM10-P1ABX
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- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | BCL2, 2210R |
| Immunogen | Recombinant full-length human BCL-2 protein (amino acids 1–239) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): Incubate antibody for 30 minutes at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death.
Members of the BCL-2 family can dimerize to modulate apoptosis. For example, Bag1 forms a heterodimer with BCL-2, enhancing its anti-apoptotic effect.
Bax and Bak play critical roles in cytochrome c release from mitochondria and initiation of apoptosis. Bax exerts a pro-apoptotic effect.
Constitutive expression of BCL2, such as from translocation to the Ig heavy chain locus, is associated with follicular lymphoma.
In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas normal or hyperplastic germinal centers are negative.
Two transcript variants of BCL-2 differ in their C-terminal ends. Overexpression of BCL-2 has been linked to cancers such as B-cell lymphoma and prostate cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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