Thermo Fisher Scientific CD4/CD8 Antibody Cocktail, FITC, PE

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

-

Product Specifications

Species Reactivity

Dog

Host/Isotype

Rat / IgG1/IgG2a

Class

Cocktail

Type

Antibody

Clone

YKIX302.9/YCATE55.9

Immunogen

Canine Concanavilin A activated T-cell blasts, Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Lyophilized

Concentration

Conc. Not Determined

Purification

purified

Storage buffer

PBS with 1% BSA, 5% sucrose

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538463

Product Specific Information

MA5-16990 contains a monoclonal mixture of FITC-conjugated CD4 (clone #YKIX 302.9, Rat IgG2a) and RPE-conjugated CD8 (clone #YCATE 55.9, Rat IgG1). This antibody is a dual-color reagent recognizing canine CD4 and CD8 cell surface antigens. Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils. Clone YCATE 55.9 recognizes CD8 alpha, which is expressed by cytotoxic T cells.

Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Target Information

CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.