Thermo Fisher Scientific EGF Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR.

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine EGF. Anti-Murine EGF-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect mEGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Murine EGF (500-P174G) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant mEGF.

Western Blot:
To detect mEGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used in conjunction with compatible secondary reagents, the detection limit for Recombinant mEGF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Packaging:
500-P174GBT-1MG will be provided as 2 × 500 µg.

Target Information

EGF (Epidermal Growth Factor) binds to the EGF receptor (EGFR), a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to environmental stimuli. Upon EGF binding, EGFR forms homo- or heterodimers with other family members, each initiating distinct signaling cascades through recruitment of SH2-containing effector proteins.

EGF is expressed in kidney, cerebrum, prostate, and salivary glands, and acts as a potent mitogenic factor. The phosphorylated receptor recruits adapter proteins such as GRB2, activating downstream cascades including:

• RAS-RAF-MEK-ERK
• PI3K-AKT
• PLCγ-PKC
• STAT modules

Research suggests EGF also plays a role in NF-κB signaling. Defects in the EGF gene are linked to hypomagnesemia type 4, and dysregulation is associated with certain cancers.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.