Thermo Fisher Scientific EGF Polyclonal Antibody, PeproTech

Applications

Western Blot (WB)

• Tested Dilution: 0.1–0.2 µg/mL

ELISA

• Tested Dilution: 0.5–2.0 µg/mL

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR

Preparation:
Produced from sera of goats immunized with highly pure Recombinant Murine EGF. Anti-Murine EGF-specific antibody was purified by affinity chromatography employing an immobilized Murine EGF matrix.

Sandwich ELISA:
To detect Murine EGF by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine EGF (500-P174GBt) as a detection antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Murine EGF.

Western Blot:
To detect Murine EGF by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Murine EGF is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Target Information

EGF (Epidermal Growth Factor) binds to the EGF receptor (EGFR), a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to environmental stimuli. Upon EGF binding, EGFR forms homo- or heterodimers with other family members, triggering distinct signaling cascades via recruitment of SH2-containing effector proteins.

EGF is widely expressed in kidney, cerebrum, prostate, and salivary glands. It acts as a potent mitogenic factor, activating downstream signaling pathways such as:

• RAS-RAF-MEK-ERK
• PI3K-AKT
• PLCγ-PKC
• STAT

EGF may also influence NF-κB signaling. Mutations in the EGF gene are associated with hypomagnesemia type 4, and dysregulation is linked to cancer progression.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.