Thermo Fisher Scientific CD14 Monoclonal Antibody (VPM65)
양 CD14 항원을 인식하는 Mouse monoclonal antibody (clone VPM65). WB, IHC(F), Flow, ELISA, IP 등 다양한 응용에 사용 가능. 단백질 A로 정제된 액상형 항체로 1 mg/mL 농도. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution / Condition |
|---|---|
| Western Blot (WB) | - |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent |
| Flow Cytometry (Flow) | Assay-dependent |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Sheep |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | VPM65 |
| Immunogen | Ovine macrophages |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.09% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2745250 |
Product Specific Information
Mouse Anti-Sheep CD14 antibody, clone VPM65 recognizes ovine CD14, a GPI-anchored 55 kDa membrane glycoprotein and monocyte/macrophage differentiation antigen belonging to the lipopolysaccharide receptor family.
Target Information
CD14 is a 55 kDa GPI-anchored glycoprotein expressed on mature monocytes, macrophages, and neutrophils. It functions as a multifunctional lipopolysaccharide receptor and exists in both membrane-bound and soluble forms. CD14 binds lipopolysaccharide via lipopolysaccharide-binding protein (LBP) and plays a key role in immune response modulation, including apoptotic cell recognition and inflammatory regulation. Alternative splicing produces multiple transcript variants encoding the same isoform. CD14 dysfunction has been associated with mycobacterium chelonae infection and croup.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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