Thermo Fisher Scientific B7-H3 (CD276) Monoclonal Antibody (M045)
인체 B7-H3(CD276)을 특이적으로 인식하는 Mouse monoclonal antibody(M045). Western blot, ICC/IF, ELISA, IP에 사용 가능. Protein G 정제, 0.25 mg/mL 농도, PBS/BSA/glycerol buffer. 다양한 암세포주에서 B7-H3 검출에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution | References |
|---|---|---|
| Western Blot (WB) | 1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:100 | - |
| ELISA | Assay-dependent | - |
| Immunoprecipitation (IP) | 1:100 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M045 |
| Immunogen | Clone M045 was generated from a recombinant protein including the extracellular region of human B7-H3. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.25 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA, 50% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2942630 |
Product Specific Information
Clone M045 mouse monoclonal antibody detects a 100–120 kDa protein on SDS-PAGE under native or denatured conditions in human LNCaP, MCF-7, NCI-H28, A431, and MDA-MB-231 carcinoma cell lines.
This antibody detects B7-H3 in membranes and cytoplasm of MCF7 cells by immunocytochemical labeling.
It is validated for use in Western blot, immunoprecipitation, immunocytochemistry, and ELISA capture assays.
Target Information
CD276 (B7-H3) is a member of the B7 family of co-stimulatory molecules. It is a type I transmembrane protein that promotes proliferation of CD4⁺ and CD8⁺ T cells, enhances cytotoxic T cell generation, and selectively stimulates interferon gamma production.
CD276 expression can be induced on dendritic cells and monocytes by inflammatory cytokines and is also expressed in peripheral tissues such as heart, kidney, testis, and colon.
In humans, CD276 exists as two isoforms resulting from gene duplication and differential splicing.
CD276 is implicated in cancer immunoregulation and has therapeutic potential, particularly when combined with anti-angiogenic therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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