Thermo Fisher Scientific CD59 Monoclonal Antibody (M011)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Clone M011 detects 15–20 kDa bands corresponding to CD59 on SDS-PAGE immunoblots of native human MDA-MB-231, A549, A431, and MCF7 cell lysates, as well as recombinant human CD59 protein.
The antibody does not detect denatured CD59.
It can be used for multiple applications including ELISA capture, western blot, immunocytochemical labeling, and immunoprecipitation.
In addition, the antibody labels live, unfixed A549 and MeWo cells.

Target Information

CD59 (Protectin) is a small (18–20 kDa) GPI-anchored, ubiquitously expressed inhibitor of the membrane attack complex (MAC).
It is a key regulator that preserves autologous cells from the terminal effector mechanism of the complement cascade.
CD59 associates with the C5b-8 complex and prevents formation of the cytolytic pore within the plasma membrane.
Additionally, CD59 acts as a low-affinity ligand of human CD2, contributing to T-cell costimulation and lymphocyte signal transduction.
Mutations in CD59 cause CD59 deficiency, characterized by hemolytic anemia, thrombosis, and cerebral infarction.
Multiple alternatively spliced transcript variants of CD59 have been identified.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.