Thermo Fisher Scientific Annexin V Monoclonal Antibody (M017)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunoprecipitation (IP)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

M017

Immunogen

Clone M017 was generated from a proprietary antigen related to human Annexin A5 in the MDA-MB-231 breast cancer cell line.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

~0.5 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2942628

Product Specific Information

Clone M017 detects a 35 kDa* protein corresponding to the molecular mass of Annexin A5 on SDS-PAGE immunoblots of human A431, MDA-MB-231, MeWo, and PC3 cells, as well as human skin, breast, lung, and brain. The antibody works for ELISA, western blot, and immunoprecipitation. In addition, mass spectrometry analysis of immunoprecipitates using AM0171 in human MDA-MB-231 cell lysates confirms this antibody only detects Annexin A5 protein.

Target Information

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.