Thermo Fisher Scientific Bcl-X (Apoptosis Marker) Monoclonal Antibody (SPM165)
Apoptosis 관련 Bcl-X 단백질을 검출하는 Thermo Fisher Scientific의 단클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. Human, Mouse, Pig, Rat 반응성. Protein A/G 정제, PBS 버퍼, 보존제 없음.
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- 598-MSM2X-P1ABX
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Pig, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM165 |
| Immunogen | Synthetic peptide aa 3–14 (Cys-QSNRELVVDFLS) of human Bcl-X protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by the BCL-X gene belongs to the BCL-2 protein family. These proteins form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in various cellular activities. They are located at the outer mitochondrial membrane and regulate the mitochondrial channel (VDAC) opening, influencing membrane potential, reactive oxygen species production, and cytochrome C release — all key factors in apoptosis. Alternative splicing produces two isoforms: the longer isoform inhibits apoptosis, while the shorter isoform promotes it.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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