Thermo Fisher Scientific GORAB Monoclonal Antibody (OTI4A4), TrueMAB
Human GORAB 단백질을 인식하는 마우스 단일클론 항체로, Western blot과 유세포분석에 사용 가능. 동결건조 형태로 제공되며, 재구성 후 약 1 mg/mL 농도로 사용. 연구용으로 적합하며, 고순도의 친화 크로마토그래피 정제 제품.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4A4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 35–394 of human GORAB produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
TCEB1, also known as protein elongin C, is a subunit of the transcription factor B (SIII) complex, which consists of elongins A/A2, B, and C.
This complex enhances elongation by RNA polymerase II by preventing transient pausing of the polymerase during transcription.
Elongin A serves as the active component, while elongins B and C are regulatory subunits.
Elongin A2 is testis-specific and forms stable complexes with elongins B and C.
The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, thereby inhibiting transcription elongation.
Western blot validation using two different antibodies against unique regions of this protein confirmed consistent molecular weight results.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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