Thermo Fisher Scientific UBE2J1 Monoclonal Antibody (OTI2G2), TrueMAB
Thermo Fisher Scientific의 UBE2J1 단클론 항체(OTI2G2)는 인간 단백질을 인식하며 Western blot과 Flow cytometry에 적합합니다. 무보존제, 동결건조 형태로 제공되며 재구성 후 1 mg/mL 농도로 사용 가능합니다. 연구용으로 ER 관련 단백질 분해 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G2 |
| Immunogen | Full length human recombinant protein of human UBE2J1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
The modification of proteins with ubiquitin is a key cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves three classes of enzymes:
- Ubiquitin-activating enzymes (E1s)
- Ubiquitin-conjugating enzymes (E2s)
- Ubiquitin-protein ligases (E3s)
UBE2J1 encodes an E2 ubiquitin-conjugating enzyme located in the endoplasmic reticulum membrane, contributing to ER-associated degradation via the ubiquitin-proteasome system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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