Thermo Fisher Scientific ASGR1 Monoclonal Antibody (8D7)
ASGR1 단백질을 검출하는 마우스 모노클론 항체(클론 8D7)로, 인간 및 랫트 시료에 반응합니다. ELISA, Western blot, IHC, Flow cytometry 등 다양한 응용에 적합하며, 단백질 G 정제 및 PBS/BSA 버퍼에 보존됩니다. 연구용으로만 사용 가능합니다.
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Thermo Fisher Scientific ASGR1 Monoclonal Antibody (8D7)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:50 | - |
| Immunohistochemistry (IHC) | - | 1 publication |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50 | - |
| Immunocytochemistry (ICC/IF) | - | 2 publications |
| Flow Cytometry (Flow) | 1:50 | 1 publication |
| ELISA | 1:50 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 8D7 |
| Immunogen | Crude rat liver membrane extracts |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 0.1% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2059674 |
Product Specific Information
- MA1-40244 detects ASGPR from rat and human samples.
- Successfully used in ELISA, flow cytometry, immunohistochemistry (frozen), immunofluorescence, and Western blot applications.
- Typical starting working dilution: 1:10.
- Immunogen: crude rat liver membrane extracts.
- Positive control: rat hepatocytes.
- Negative control: rat kidney, pancreas, small intestine, colon.
Target Information
The asialoglycoprotein receptor (ASGPR) is a transmembrane glycoprotein (42 kDa) that mediates binding, internalization, and degradation of extracellular glycoproteins with exposed terminal galactose residues. It is expressed on hepatocyte surfaces in a polar manner—present on sinusoidal and lateral membranes but absent on bile canalicular membranes.
The mammalian hepatic ASGPR mediates endocytosis and degradation of serum proteins lacking terminal sialic residues.
Rat ASGPR consists of three subunits: RHL-1, RHL-2, and RHL-3.
Monoclonal antibody 8D7 recognizes a subunit-specific epitope of RHL-1 and is cross-reactive with human ASGPR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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