Thermo Fisher Scientific NMDAR2A Monoclonal Antibody (N327/95), FITC
FITC 형광 표지된 NMDAR2A 단클론 항체로 인간, 생쥐, 랫트 시료에 반응. WB, IHC, ICC 등 다양한 응용 가능. GluN2A/NR2A 단백질 검출에 특이적이며 NR2B에는 반응하지 않음. 단백질 G 정제, 액상 형태로 제공.
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Thermo Fisher Scientific NMDAR2A Monoclonal Antibody (N327/95), FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N327/95 |
| Immunogen | Fusion protein amino acids 75–325 (extracellular N-terminus) of rat GluN2A/NR2A |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932055 |
Additional Formats
- Unconjugated (MA5-27692)
- APC (MA5-45600)
- PE (MA5-45603)
- PerCP (MA5-45602)
- Request custom conjugation (Thermo Fisher form)
Product Specific Information
- 1 µg/mL of MA5-45601 was sufficient for detection of GluN2A/NR2A in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as secondary antibody.
- Detects approximately 170 kDa.
- Does not react with NR2B.
- This antibody was formerly sold as clone S327-95.
Target Information
N-methyl-D-aspartate (NMDA) receptors are ionotropic glutamate-gated ion channels involved in long-term potentiation, a process underlying memory and learning.
NMDA receptor channels are heteromers composed of NMDAR1 (GRIN1) and one or more of the NMDAR2 subunits: NMDAR2A (GRIN2A), NMDAR2B (GRIN2B), NMDAR2C (GRIN2C), and NMDAR2D (GRIN2D).
Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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