Thermo Fisher Scientific H1FOO Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:500–1:2,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total Histone H1.

Target Information

Eukaryotic histones are basic, water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA into a left-handed super-helical turn, forming chromosomal fibers.
Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, consisting of two H2A-H2B dimers and two H3-H4 dimers with nearly symmetrical tertiary structures.
Over 80% of nucleosomes contain the linker histone H1, derived from an intronless gene, which interacts with linker DNA between nucleosomes and mediates chromatin compaction.
H1FOO (H1 histone family, member O, oocyte-specific), also known as OSH1, is a 346-amino-acid oocyte-specific histone localized to both the nucleus and cytoplasm. Expressed as multiple alternatively spliced isoforms, H1FOO plays an important role in gene regulation during oogenesis and early embryogenesis and is crucial for meiotic maturation of germinal vesicle-stage oocytes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.