Thermo Fisher Scientific FH Monoclonal Antibody (OTI1F10), TrueMAB
FH 단백질을 인식하는 OTI1F10 클론의 Mouse IgG1 단일클론 항체. WB, IHC, ICC, Flow 등 다양한 응용에 사용 가능. HEK293T 세포에서 생산된 인간 FH 재조합 단백질을 면역원으로 사용. 고순도 친화 크로마토그래피 정제, 무보존제 PBS 버퍼에 동결건조 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F10 |
| Immunogen | Full length human recombinant FH protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The FH (Fumarase) protein is an enzymatic component of the tricarboxylic acid (Krebs) cycle, catalyzing the conversion of fumarate to L-malate. It exists in both cytosolic and mitochondrial forms, differing by the translation start site. The mitochondrial form loses its N-terminal extension to become identical to the cytoplasmic form. FH functions as a homotetramer and mutations in this gene can cause fumarase deficiency, leading to progressive encephalopathy.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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