Thermo Fisher Scientific MAP2K1 Monoclonal Antibody (OTI1C1), TrueMAB
MAP2K1(MEK1) 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 면역세포염색에 적합합니다. 고순도 친화 크로마토그래피 정제 제품이며, 동결건조 형태로 제공됩니다. 세포 성장 및 분화 신호전달 연구용으로 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1C1 |
| Immunogen | Full-length protein expressed in 293T cells transfected with human MAP2K1 expression vector |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
MAP2K1 (MEK1) is a dual-specificity protein kinase functioning in the MAPK cascade, regulating cell growth and differentiation. It is activated by various growth factors, cytokines, and calcium influx. MEK1 activation occurs through phosphorylation at serine residues 218 and 222 by Raf1. It plays roles in stress responses, apoptosis, and cytokine-induced proliferation. Mutations in MAP2K1 can lead to cardiofaciocutaneous syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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