Thermo Fisher Scientific BuB1B Monoclonal Antibody (OTI6E3), TrueMAB
BuB1B 단클론 항체(OTI6E3)는 인간, 개, 쥐에서 반응하며 Western Blot, ICC/IF, Flow Cytometry에 적합합니다. 정제된 무보존 항체로, 재구성 후 1 mg/mL 농도로 사용 가능합니다. 세포주기 체크포인트 연구 및 암 관련 단백질 분석에 유용합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6E3 |
| Immunogen | Full length human recombinant protein of human BUB1B produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
BUBR1 is a Ser/Thr protein kinase involved in the mitotic checkpoint, delaying anaphase until chromosomes are properly attached to the spindle. Defects in this checkpoint contribute to genetic instability and cancer progression.
Human cells contain two checkpoint kinases derived from the BUB1 gene: hBUBR1 and hBUB1. Both monitor kinetochore-microtubule interactions, ensuring proper mitotic progression.
BUBR1 has been detected in human cancers and is known to interact with BCSG1, potentially accelerating breast cancer progression by compromising checkpoint control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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