Thermo Fisher Scientific 6X His Tag Monoclonal Antibody (33D10.D2.G8), DyLight 488
6X His 태그 단백질 검출용 DyLight 488 결합 단클론 항체. Western blot, ICC/IF, ELISA 등에 사용 가능. 높은 특이성과 재현성 제공. 단백질 정제 및 발현 확인에 적합. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | >1:10,000–1:25,000 |
| Immunocytochemistry (ICC/IF) | >1:5,000 |
| ELISA | >1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 33D10.D2.G8 |
| Immunogen | This antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide. |
| Conjugate | DyLight™ 488 |
| Excitation/Emission Max | 492 / 519 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats Available
- Unconjugated
- Atto 550
- Atto 647N
- Biotin
- Cyanine3
- Cyanine5
- DyLight 405
- DyLight 549
- DyLight 649
- DyLight 680
- DyLight 800
- HRP
- Custom conjugation upon request
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid freeze/thaw cycles. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is directed against the 6X His motif and is useful in determining its presence in various assays. It detects overexpressed proteins containing the 6X His epitope tag and has reacted with all His-tagged proteins tested. In western blotting of bacterial extracts, it does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy-termini of targeted proteins in transfected or transformed cells.
Target Information
The 6X His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to facilitate isolation or purification via immobilized metal affinity chromatography. Epitope tagging allows detection of recombinant proteins even when no specific antibody exists. His-tagged proteins bind strongly to Ni²⁺ ions, enabling purification to homogeneity using Ni²⁺-NTA affinity chromatography. His tag-specific antibodies, such as this one, are valuable for detection or co-immunoprecipitation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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