Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 615
• Brilliant UV 661
• Brilliant UV 737
• Brilliant UV 805
• Brilliant Violet 421
• Brilliant Violet 480
• Brilliant Violet 650
• Brilliant Violet 786
• PE
• PE-Cyanine5
• PE-Cyanine7
• PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Super Bright 702
• Super Bright 780
• Request custom conjugation available

Product Specific Information

The J105 monoclonal antibody reacts with human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 plays a key role in peripheral tolerance and autoimmune disease and is expressed predominantly on activated T and B lymphocytes. Its ligands PD-L1 (B7-H1) and PD-L2 (B7-DC) show overlapping functions and are expressed on normal and activated antigen-presenting cells.

Costaining experiments indicate eBioJ105 recognizes a different epitope than MIH4 (Product #11-9969).

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested by flow cytometry on normal human peripheral blood cells at 5 µL (0.5 µg)/test (100 µL final volume, 10⁵–10⁸ cells/test).

Brilliant Violet™ 711 (BV711):

• Tandem dye emitting at 713 nm for use with 405 nm violet laser.
• Use Super Bright Complete Staining Buffer (Product #SB-4401-42) or Brilliant Stain Buffer™ (Product #00-4409-75) to minimize non-specific polymer interactions.
• Sensitive to light; protect vial and stained samples.
• Fixation compatibility: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) up to 3 days at 4°C in dark.
• Not compatible with methanol-based fixation.

Excitation: 407 nm
Emission: 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

T-cell activation is regulated by PD-1, which delivers inhibitory signals upon binding to its ligands PD-L1 and PD-L2 on antigen-presenting cells. PD-1 deficiency leads to hyperactivated immune responses and autoimmune disease. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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