Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Violet 711, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.5 µg)/test

Product Specifications

Additional Formats Available

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661 / 737
• Brilliant Violet 421 / 480 / 786
• FITC
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 700
• PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 780
• Request custom conjugation

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), a G-protein-coupled chemokine receptor functioning as a receptor for CCL19 and CCL21. CCR7 is expressed on T cells and dendritic cells (DCs), distinguishing naive, central, and effector memory T cell populations. It plays a key role in migration and DC maturation processes including chemotaxis, endocytosis rate, survival, and cellular architecture.

• Recommended incubation: ≥45 minutes at 2–8°C for optimal staining
• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on normal human peripheral blood cells
• Usage: 5 µL (0.5 µg) per test (100 µL final volume, 10⁵–10⁸ cells/test)

Fluorochrome Information:
Brilliant Violet™ 711 (BV711) emits at 713 nm and is suitable for cytometers with a 405 nm violet laser. Protect samples from light due to photo-induced oxidation sensitivity.

Buffer Recommendations:
Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to minimize non-specific polymer interactions when using multiple polymer dye-conjugated antibodies.

Fixation Guidance:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54). BV711 is not compatible with methanol-based fixation.

Excitation/Emission:

• Excitation: 407 nm
• Emission: 713 nm
• Laser: Violet Laser

Trademark Notice:
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

CCR7 belongs to the G protein–coupled receptor family and is induced by Epstein-Barr virus (EBV). It mediates EBV effects on B lymphocytes and is expressed in blood, bone marrow, lymph nodes, and intestines. CCR7 regulates migration of memory T cells and dendritic cell maturation. Its specific ligands include CCL19/ECL. Other related receptors include CCR1–CCR5 and the Duffy antigen, all involved in chemotaxis and immune cell migration.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지