Thermo Fisher Scientific Cyanine Polyclonal Antibody, Biotin
Cyanine 계열 형광 염료(Cy2, Cy3, Cy5 등)에 반응하는 Biotin 결합 PolyClonal 항체입니다. Western blot 및 ELISA에 적합하며, 높은 특이성과 안정성을 제공합니다. 장기 보관 시 -20°C에서 보관 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
ELISA
- Tested Dilution: 1:8,000–1:40,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host/Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A mixture of Cy3 and Cy5 conjugated KLH |
| Conjugate | Biotin |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | 0.02M potassium phosphate, pH 7.2, with 10 mg/mL BSA, 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.
Target Information
Cyanines are blue-green fluorescent dyes belonging to the polymethine group, used in biotechnology for labeling and analysis.
The spectrum for Cyanine ranges from IR (489/506) to UV (743/767). Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, and Cy7.
These dyes exhibit brighter and more stable fluorescence than traditional FITC, TRITC/Rhodamine dyes. Cy3 and Cy5 are most commonly used for two-color detection in Flow Cytometry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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