Thermo Fisher Scientific Cyanine Polyclonal Antibody, Biotin

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

ELISA

• Tested Dilution: 1:8,000–1:40,000

Product Specifications

Product Specific Information

Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Sheep Serum and cyanine conjugated IgG. Reactivity was observed against Cy2, Cy3 and Cy5. Reactivity against other cyanine analogs is unknown.

Target Information

Cyanines are blue-green fluorescent dyes belonging to the polymethine group, used in biotechnology for labeling and analysis.
The spectrum for Cyanine ranges from IR (489/506) to UV (743/767). Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, and Cy7.
These dyes exhibit brighter and more stable fluorescence than traditional FITC, TRITC/Rhodamine dyes. Cy3 and Cy5 are most commonly used for two-color detection in Flow Cytometry applications.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.