Thermo Fisher Scientific AKT Polyclonal Antibody
AKT 단백질을 인식하는 Thermo Fisher Scientific의 Sheep Polyclonal Antibody. Western blot, IHC, ELISA, IP 등 다양한 응용에 적합. 인간 AKT1에 반응하며, 쥐, 생쥐, 닭에서도 교차 반응 예상. 고순도 친화 크로마토그래피 정제 및 안정한 액상 형태.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:10,000–1:25,000 |
| Immunohistochemistry (IHC) | Assay-dependent |
| ELISA | 1:5,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Anti-AKT antibody was affinity purified and prepared by repeated immunizations with a synthetic peptide corresponding to an internal region of human AKT1 protein. A cysteine residue was added to facilitate coupling. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.9 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is directed against AKT and reacts with AKT from human tissues. Based on sequence homology, it is expected to react with rat, mouse, and chicken AKT as well.
Target Information
AKT, also known as protein kinase B (PKB) or RAS-alpha, is a ubiquitous serine/threonine kinase involved in various biological responses such as regulation of metabolism, cell survival, and growth by phosphorylating multiple proteins.
It is activated by insulin, PI3K, IGF1, and other growth and survival factors. AKT promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors and caspase-9.
The AKT pathway is a major target for cancer drug discovery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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