Thermo Fisher Scientific Cyanine Polyclonal Antibody, HRP
Western blot 및 ELISA에 적합한 HRP 결합 Sheep polyclonal 항체. Cy3 및 Cy5 결합 KLH를 면역원으로 사용. 높은 특이성과 안정성, 장기 보관 가능. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
ELISA
- Tested Dilution: 1:1,500–1:6,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A mixture of Cy3 and Cy5 conjugated KLH |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02 M potassium phosphate (pH 7.2), 0.15 M NaCl, 10 mg/mL BSA |
| Contains | 0.01% gentamicin sulfate |
| Storage Conditions | Store at 4°C (short term); for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Store vial at 4°C prior to restoration.
- For extended storage, aliquot contents and freeze at -20°C or below.
- Avoid repeated freeze/thaw cycles.
- Centrifuge product if not completely clear after standing at room temperature.
- Stable for several weeks at 4°C as an undiluted liquid.
- Dilute only immediately prior to use.
Assay Results:
Immunoelectrophoresis showed a single precipitin arc against anti-Sheep Serum, anti-Peroxidase, and cyanine-conjugated IgG.
Reactivity observed against Cy2, Cy3, and Cy5. Reactivity with other cyanine analogs is unknown.
Target Information
Cyanines are blue-green fluorescent dyes of the polymethine group, widely used in biotechnology for labeling and analysis.
Their spectrum ranges from IR (489/506) to UV (743/767).
Cyanine dyes include Cy2, Cy3, Cy3B, Cy3.5, Cy5, Cy5.5, and Cy7, offering brighter and more stable fluorescence than FITC or TRITC/Rhodamine dyes.
Cy3 and Cy5 are most commonly used for two-color detection in flow cytometry applications.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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