Thermo Fisher Scientific Nestin Polyclonal Antibody
Rabbit polyclonal antibody against human Nestin protein for IHC (Paraffin) applications. Recommended dilution 1:100. Useful for identifying stem/progenitor cells and tumor endothelial cells. Supplied as liquid, unconjugated form, stored at 4°C or -20°C.
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- PA532516
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- pk
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Thermo Fisher Scientific Nestin Polyclonal Antibody
Applications
- Immunohistochemistry (Paraffin) (IHC (P))
Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide derived from C-terminus of human nestin protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549982 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes.
After antigen retrieval, incubate samples with primary antibody for 30 minutes at room temperature.
A suggested positive control is liver hepatoma.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and early embryonic neuroepithelial stem cells.
It is widely used as a marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin serves as a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin help characterize progenitor cell differentiation and enhance therapeutic potential in CNS disease or injury.
Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and as a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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