Thermo Fisher Scientific MSH6 Polyclonal Antibody
MSH6 단백질을 인식하는 Rabbit Polyclonal 항체로, 인체 시료에 반응합니다. 파라핀 포매 조직의 면역조직화학(IHC-P)에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. DNA 손상 감지 및 복구 연구에 활용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide corresponding to internal region of human MSH6 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.6, with 1% BSA |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2549975 |
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10 mM citrate buffer (pH 6.0) for 10 minutes, followed by cooling at room temperature for 20 minutes. After retrieval, incubate samples with the primary antibody for 30 minutes at room temperature.
A suggested positive control is colon carcinoma tissue.
Target Information
The DNA mismatch repair MSH2/MSH6 heterodimer is responsible for detecting DNA damage and may promote apoptosis in normal cells. This mechanism helps protect mammals from ultraviolet-induced malignant transformation and contributes to the prevention of skin cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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